Práctica de Laboratorio: Biomoléculas orgánicas

Práctica de Laboratorio: Biomoléculas orgánicas

Las biomoléculas orgánicas son compuestos formados principalmente por elementos como carbono, hidrógeno, oxígeno, nitrógeno, azufre, fósforo entre otros; que solo pueden ser sintetizados por los seres vivos.

Las biomoléculas orgánicas se dividen en cinco grandes grupos: carbohidratos, lípidos, proteínas, vitaminas y ácidos nucleicos.

A continuación se presentaran algunas pruebas sencillas que puedes realizar en casa y de esta manera conocer e identificar algunas biomoléculas. 

Objetivo general: Estudiar e identificar carbohidratos, vitamina C, proteínas y enzimas en algunos alimentos.

 

Materiales y sustancias a utilizar.

  • Recipientes de vidrio
  • Cocinilla eléctrica
  • Goteros
  • Jeringa 10 mL
  • Zumo de frutas (naranja, manzana, uva, limón, etc)
  • Alcohol isopropilico o etílico
  • Huevo
  • Leche
  • Pan
  • Papa
  • Levadura
  • Azúcar
  • Almidón (fécula de maíz)
  • Solución de Lugol (Betadine)
  • Agua oxigenada de 10 y 20 volúmenes
  • Vaso medidor
Parte I. Proteínas

a) Desnaturalización de la proteína del huevo

Procedimiento:
Separa la clara de la yema de huevo. Luego en 2 recipientes de vidrio coloca la misma cantidad de clara. En el primer recipiente agrega alcohol etílico y el segundo recipiente somételo a calentamiento en un baño de María. Para ambos casos, observa lo que ocurre y responde:
• Explica ¿qué le sucede a la clara?
• ¿Cómo se manifiesta la desnaturalización de la clara de huevo?
• ¿Cuál proteína contiene la clara del huevo?
• ¿Es un proceso irreversible? Explique
• ¿Qué otro agente puede desnaturalizar la proteína de la clara de huevo?

b) Desnaturalización de la proteína de la leche.

Procedimiento:
Coloca un poco de leche en un recipiente y llévalo a calentamiento en un baño de María, sin llegar a hervir. Luego agrégale un poco de zumo de limón. Observa lo que ocurre y responde:
• Explica ¿qué le sucede a la leche?
• ¿Cómo se manifiesta la desnaturalización de la leche? ¿Qué significa que la leche está “cortada”?
• ¿Cuál proteína contiene la leche?
• ¿Es un proceso irreversible? Explique
• ¿Qué otro agente puede desnaturalizar la proteína de la leche?

Parte II. Enzimas. Acción de la catalasa.

Procedimiento 1: Agua oxigenada de 20 volúmenes y levadura

  • Necesitarás de un recipiente alto de vidrio  para que se pueda apreciar el experimento, ya que el producto formado es expulsado hacia arriba y de forma rápida.
  • Coloca en el recipiente media taza de agua oxigenada de 20 volúmenes.
  • Luego añade 10 gotas de colorante vegetal de tu preferencia (opcional).
  • Luego agrega levadura y agitas la mezcla.
  • Espera unos minutos y observa lo que ocurre.

.Procedimiento 2: Agua oxigenada y papa

En un recipiente agrega un poco de agua oxigenada de 10 volúmenes y luego añade un trozo de papa. Observa lo que ocurre.

Para ambos casos responde:

  • ¿Qué ocurre al agregar la levadura y la papa?
  • ¿Cuál reacción fue más rápida?
  • ¿Cuál es la función de la levadura y la papa?
  • ¿Qué le ocurrió al agua oxigenada?
  • ¿Qué es la catalasa?
Parte III. Carbohidratos.

Prueba de Lugol

Para este experimento necesitarás de una papa, pan, solución de sacarosa, jugo de naranja y solución de almidón. A cada muestra agrégale unas gotas de Lugol y observa lo que ocurre. Si no dispones de la solución de Lugol, puedes utilizar una solución de betadine (polividona yodada), que no es más que una solución empleada como desinfectante y antiséptico, principalmente para tratar cortes menores en la piel.

La prueba de Lugol da positivo cuando muestra una coloración púrpura o azul oscuro.

Responde:

  • ¿En qué consiste la prueba de Lugol?
  • ¿Por qué el almidón se torna purpura o azul cuando se le añade Lugol?
  • ¿Por qué la sacarosa y el jugo de naranja dan negativo para esta prueba? Explique.

 

Parte IV. Vitamina C

Se realizará una prueba para identificar la cantidad de vitamina C que hay en diferentes tipos de jugos. Para ello, primeramente se debe preparar una solución analizadora, la cual permitirá identificar la vitamina C.

Esta solución se prepara de la siguiente manera:

Disuelve una pequeña cantidad de fécula de maíz en agua y luego sométela a calentamiento. El líquido sobrenadante será el almidón en solución.

Ahora añade 10 mL de disolución de almidón a 250 mL de agua, y a esto unas gotas de betadine (yodo en disolución). Trata de que el líquido se torne azul oscuro, ya que si añades mucho yodo, el líquido quedará parduzco y el análisis de vitamina C no será efectivo.

Ahora es momento de analizar la cantidad de vitamina C que hay en los zumos de frutas que tengamos a la mano. Para ello, dispone de la cantidad de recipientes de vidrio que corresponda a los diferentes zumos que tengamos. En cada recipiente añade 10 mL de la solución azul analizadora. Luego con un gotero añade gota a gota el zumo de fruta de tu preferencia y anota en un cuadro la cantidad que se necesitó para decolorar la solución. Puedes probar con zumo natural o envasado, agua, zumo recién exprimido o que se ha calentado antes del análisis.

El zumo tendrá más vitamina C cuantas menos gotas necesitemos para decolorar la solución analizadora.

Responde:

  • ¿Qué es la vitamina C?
  • ¿Por qué se decolora la solución yodada en presencia de la vitamina C?
  • ¿Cuál de los zumos de frutas analizados posee mayor cantidad de vitamina C? ¿cuál posee menor cantidad?

 

NOTA: Existen muchísimas pruebas de laboratorio para identificar las biomoléculas, acá simplemente estamos colocando las más sencillas, es decir aquellas que se pueden hacer desde casa. 

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